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 2. PCR is a three step process

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PostSubject: 2. PCR is a three step process   14/01/09, 10:57 pm

Primer- a short segment of DNA that acts as the starting point for a new strand

Recipe for PCR-
Materials
• DNA to be copied
• DNA polymerases
• Large amounts of each of the four DNA nucleotides (A, T, G, & C)
• Two primers (DNA polymerase can add nucleotides to strands that have already been started, but it cant start its own strand) Primers are like bookends

Steps
1. SEPARATING. The container with all the reactants is heated to separate the double-stranded DNA into single strands.

2. BINDING. The container is cooled and the primers bind to each DNA strand. The primers bind on opposite ends of the DNA being copied

3. COPYING. The container is heated again and the polymerases begin to build new strands of DNA. Added nucleotides bind to the original DNA strands by complementary base pairing. The polymerases continue attaching nucleotides until the entire DNA segment has been copied.


• Each cycle doubles the amount of DNA copied, which is why it is called a chain reaction
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2. PCR is a three step process
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