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 2. Restriction Enzymes cut DNA

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PostSubject: 2. Restriction Enzymes cut DNA   06/01/09, 09:22 pm

•DNA is cut to study gene

•Restriction enzymes are used- they cut when they find specific nucleotide sequences

•The more restriction sites the restriction enzyme finds, the more pieces are cut.
More pieces = shorter length

•Nucleotide sequences 4-8 base pair long

•Cuts straight across- blunt ends

•Sometimes made to stagger cut, to make “sticky ends” that work like Velcro ready to hook onto opposite sides. Used in Biotechnology
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